Questions? Comments? Please contact Dr. Phillip McClean or Christina Johnson.

OVERVIEW
Flythrough Tour
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MOLECULAR PROCESSES
Transcription
Regulated Transcription
mRNA Processing
mRNA Splicing
Translation
Lac Operon
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CELLULAR PROCESSES
Protein Trafficking
Protein Modification
Protein Recycling
Insulin Signaling
Constitutive Secretion
Regulated Secretion
Mitochondrial Protein Transport
Mitosis
Meiosis
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CELLULAR ENERGY CONVERSION
Atp Synthase (Gradients)
Electron Transport Chain
Photosynthesis (Light Reaction)
Photosystem II
Glycolysis (Overview)
Glycolysis (Reactions)
Citric Acid Cycle (Overview)
Citric Acid Cycle (Reactions)
Energy Consumption
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HOME > mRNA splicing > ADVANCED LOOK > 1.) Primary transcript
mRNA Splicing: Advanced Look --> 1.) Primary Transcript

When mRNA is ready for splicing, it is known as a primary transcript. Clicking on each of the thumbnail images will bring up a larger, labeled version of the described scene.

To see the Flash movie for the following sequence of images, click here.

The primary transcript contains sequences known as exons and introns.

This primary transcript contains two exons and one intron. Here we see exon 1.

Here we see the intron. Introns contain sequences that guide the splicing process. These include a 5' GU (GT in DNA) sequence...

...an A branch site located near a pyrimidine-rich region...

...and a 3' AG sequence.

Exon 2 is found on the opposite end of the intron. The 3' end of the primary transcript also contains the poly-A tail added during mRNA processing.

NEXT --> 2.) SPLICEOSOME

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