VCAC: Molecular Processes: mRNA Splicing: Advanced Look: Splicing

Questions? Comments? Please contact Dr. Phillip McClean or Christina Johnson.

Flythrough Tour

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CELLULAR PROCESSES

Atp Synthase

Electron Transport Chain

Protein Trafficking

Protein Modification

Protein Recycling

Constitutive Secretion

Regulated Secretion

Mitochondrial Protein Transport

Photosynthesis

Photosystem II

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MOLECULAR PROCESSES

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NATIONAL SCIENCE FOUNDATION

U.S. DEPARTMENT OF EDUCATION

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HOME > mRNA splicing > ADVANCED LOOK > 1.) primary transcript > 2.) spliceosome > 3.) splicing

mRNA Splicing: Advanced Look --> 3.) Splicing

Splicing refers to the action by which the splicesome removes an intron and connects the remaining exons together. Clicking on each of the thumbnail images will bring up a larger, labeled version of the described scene.

To see the Flash movie for the following sequence of images, click here.

	Splicing can now begin. First, the 5' end of the intron is cleaved.
	The 5' GU end of the intron is then moved toward the A branch site, creating a lariat structure.
	Next, the 3' end of the intron is cleaved.
	Once the intron has been fully cleaved, the two exons are ligated together.
	The spliceosome then dissociates.
	Following splicing, the lariat intron quickly degrades.
	The mature strand of mRNA is now ready for translation.